Species reactivityReacts with: Mouse, Horse, Human, Monkey
Does not react with
Fruit fly (Drosophila melanogaster)
Immunogen
Recombinant fragment, corresponding to C terminal amino acids 291-726 of Human Cyclin T1
General notes
ab27963 will also recognise truncated forms of cyclin T1 which are frequently produced by proteolysis during sample preparation.
Properties
FormLiquid
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
PurityWhole antiserum
Primary antibody notes ab27963 will also recognise truncated forms of cyclin T1 which are frequently produced by proteolysis during sample preparation.
Clonality Polyclonal
IsotypeIgG
Application notesEMSA: Use at an assay dependent dilution PMID 17576689. IP: Use at an assay dependent dilution. WB: 1/1000. Detects a band of approximay 87 kDa (predicted molecular weight: 81 kDa).
Not yet tested in other applications. Optimal dilutions/concentrations should be determined by the end user.
Target
FunctionRegulatory subunit of the cyclin-dependent kinase pair (CDK9/cyclin-T1) complex, also called positive transcription elongation factor B (P-TEFb), which is proposed to facilitate the transition from abortive to productive elongation by phosphorylating the CTD (carboxy-terminal domain) of the large subunit of RNA polymerase II (RNA Pol II). In case of HIV or SIV infections, binds to the transactivation domain of the viral nuclear transcriptional activator, Tat, thereby increasing Tat's affinity for the transactivating response RNA element (TAR RNA). Serves as an essential cofactor for Tat, by promoting RNA Pol II activation, allowing transcription of viral genes.
Tissue specificityUbiquitously expressed.
Sequence similaritiesBelongs to the cyclin family. Cyclin C subfamily.
Cellular localizationNucleus.
Anti-Cyclin T1 antibody images
Western blot - Cyclin T1 antibody (ab27963)
All lanes : Anti-Cyclin T1 antibody (ab27963) at 1/1000 dilution Lane 1 : HeLa whole cell lysate Lane 2 : HeLa cell nuclear lysate Lane 3 : monkey Vero cell lysate Lane 4 : mouse kidney cell lysate Lane 5 : Drosophila S2 cell lysate Lane 6 : horse dermal cell lysate Secondary donkey anti-sheep peroxidase conjugate at 1/20000 dilution Predicted band size : 81 kDa Observed band size : 87 kDa
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