現(xiàn)貨供應(yīng)，歡迎訂購: :CHO HCP ELISA kit, 3G This CHO HCP 3rd Generation ELISA kit uses a new and even more broadly reactive antibody than our earlier kits. The kit incorporates a number of other improvements in the detection of CHO HCPs. This new ELISA is more sensitive than the previous kits, able to detect HCPs in the range of 100 parts per billion for a variety of antibodies and other therapeutic proteins expressed in CHO. It has been extensively qualified with a large number of drug substances and in-process samples. This data indicates the kit is truly a generic method that can likely be validated as not only a process development tool, but in most cases as a lot release test, eliminating the need to develop a custom or process specific assay. The antibody has been evaluated for reactivity to individual HCPs using the conventional 2D Western Blot as well as a method we have shown to be superior to 2D WB, which we call 2D HPLC-ELISA. This 2D HPLC-ELISA method demonstrated the antibody is reactive to more than 750 individual HCPs from conditioned media and cell lysates in both a CHO-S strain as well as a K1 strain. These 750+ HCPs represent more than 98% of the total mass of protein as indicated by protein detection methods orthogonal to ELISA. As such, this antibody may overcome concerns about missing any significant individual HCPs. This new ELISA is also more robust than the earlier kits showing less sample matrix interference and improved dilutional linearity. If you have found the F015 and CM015 kits lacking in any way meeting your HCP detection needs, or if you are considering the need for a process-specific assay, we recommend you evaluate this 3rd Generation CHO HCP ELISA kit. Host Cells used for recombinant expression are complex systems containing hundreds to thousands of host cell proteins (HCPs) that can contaminate biopharmaceutical products. Failure to sufficiently remove contaminants early in drug development can result in reduced drug efficacy or adverse patient reactions. Regulations require HCPs be reduced to the lowest levels possible. Our generic ELISA kits are useful in both process development and process validation. Using these kits in the earliest phase of product development can provide relevant and specific data directing process improvement and decreasing the levels of HCP contamination. In quality control applications, subject to thorough validation and qualification by each laboratory, these kits may also be useful for lot release testing. 相關(guān)產(chǎn)品： Product Catalog # Description A549 HCP ELISA kit F230 Host cell protein assay, 96 tests A549 HCP Western Blot Kit F235 Host cell protein assay, 5 mini blots BHK HCP ELISA Kit F510 Host cell protein assay, 96 tests Bovine IgG ELISA Kit F070 Bioprocess contaminant assay, 96 tests Bovine Plasma Protein ELISA Kit F290 Bioprocess contaminant assay, 96 tests BSA ELISA Kit F030 Bioprocess contaminant assay, 96 tests Bovine Transferrin ELISA Kit F120 Bioprocess contaminant assay, 96 tests CHO HCP ELISA Kit F015 Host cell protein assay, 96 tests CHO HCP Western Blot Kit F060 Host cell protein assay, 5 mini blots CHO-CM ELISA Kit CM015 Host cell protein assay, 96 tests CHO-CM Western Blot Kit CM060 Host cell protein assay, 5 mini blots CHO HCP ELISA Kit, 3G F550 Host cell protein assay, 96 tests E. coli HCP ELISA Kit F410 Host cell protein assay, 96 tests E. coli HCP Western Blot Kit F415 Host cell protein assay, 5 mini blots Goat Casein HCP ELISA Kit F420 Host cell protein assay, 96 tests Goat IgG ELISA Kit F210 Bioprocess contaminant assay, 96 tests Total Goat Milk Proteins ELISA kit F240 Host cell protein assay, 96 tests Total Goat Milk Proteins WB kit F245 Host cell protein assay, 5 mini blots Human Albumin ELISA Kit F055 Bioprocess contaminant assay, 96 tests Human IgA ELISA Kit F165 Bioprocess contaminant assay, 96 tests Human IgG-total ELISA Kit F160 Bioprocess contaminant assay, 96 tests Human IgM ELISA Kit F170 Bioprocess contaminant assay, 96 tests HEK 293 HCP ELISA Kit F650 Host cell protein assay, 96 tests Human Transferrin ELISA Kit F035N Bioprocess contaminant assay, 96 tests Insect Cell SF9 HCP ELISA Kit F020 Host cell protein assay, 96 tests Insect Cell SF9 Western Blot Kit F125 Host cell protein assay, 5 mini blots Insulin ELISA Kit F040 Bioprocess contaminant assay, 96 tests Lactococcus lactic HCP ELISA Kit F490 Host cell protein assay, 96 tests Lactococcus lactic HCP WB Kit F495 Host cell protein assay, 5 mini blots Mouse Total IgG ELISA Kit F049 Bioprocess contaminant assay, 96 tests Mouse IgG1 ELISA Kit F045 Bioprocess contaminant assay, 96 tests Mouse IgG2a ELISA Kit F046 Bioprocess contaminant assay, 96 tests Mouse IgG2b ELISA Kit F047 Bioprocess contaminant assay, 96 tests Mouse IgM ELISA Kit F090 Bioprocess contaminant assay, 96 tests MRC5 HCP ELISA Kit F300 Host cell protein assay, 96 tests NS/0 HCP ELISA kit F220 Host cell protein assay, 96 tests NS/0 HCP Western Blot kit F225 Host cell protein assay, 5 mini blots PER.C6 cell line HCP ELISA Kit F530 Host cell protein assay, 96 tests Pichia pastoris HCP ELISA Kit F140 Host cell protein assay, 96 tests P.pastoris HCP Western Blot Kit F145 Host cell protein assay, 5 mini blots Pichia pastoris HCP ELISA Kit, 2nd Generation F640 Antibodies to glycosylation epitopes have been removed and is much more specific than the F140. Protein A ELISA Kit F050 Bioprocess contaminant assay, 96 tests Protein A ELISA for human IgG F050H Bioprocess contaminant assay, 96 tests Protein A ELISA kit F400 Bioprocess contaminant assay, 96 tests Ps. fluorescens HCP ELISA kit F450 Host cell protein assay, 96 tests S. cerevisiae HCP ELISA Kit F135 Host cell protein assay, 96 tests S. cerevisiae HCP Western Blot Kit F130 Western Blot assay, 5 mini blots SP 2/0 HCP ELISA Kit F180 Host cell protein assay, 96 tests SP 2/0 HCP Western Blot Kit F185 Western Blot assay, 5 mini blots S.aureus HCP ELISA Kit F320 Host cell protein assay, 96 tests Ultra-Sensitive Insulin ELISA Kit F280 Bioprocess contaminant assay, 96 tests Vero cell HCP ELISA kit F500 Host cell protein assay, 96 tests Polyclonal Antisera A549 HCP A549 Goat, neat serum A549 HCP A549-PA Goat, IgG fraction A549 HCP A549-AF Goat, affinity purified Bovine Plasma Proteins BPP04 Rabbit, neat serum Bovine Plasma Proteins BPP04-PA Rabbit, IgG fraction Bovine Plasma Proteins BPP04-AF Rabbit, affinity purified BHK HCP BHK1107 Goat, neat serum BHK HCP BHK1107-PA Goat, IgG fraction BHK HCP BHK1107-AF Goat, affinity purified Campylobacter species 9-25B-PA Rabbit, IgG fraction Campylobacter species 9-61A Guinea Pig, neat serum Chlamydia species 8-29A Rabbit, neat serum Chlamydia species 8-29A-PA Rabbit, IgG fraction Chlamydia species 8-48 Guinea Pig, neat serum CHO HCP C0016 Goat, neat serum CHO HCP C0016-PA Goat, IgG fraction CHO HCP C0016-AF Goat, affinity purified CHO-CM HCP M0016 Goat, neat serum CHO-CM HCP M0016-PA Goat, IgG fraction CHO-CM HCP M0016-AF Goat, affinity purified CHO HCP 3G 3G-0016 Goat, neat serum CHO HCP 3G 3G-0016PA Goat, IgG fraction CHO HCP 3G 3G-0016AF Goat, affinity purified Cytomegalovirus 8-21B Rabbit, neat serum Cytomegalovirus 8-53A Guinea Pig, neat serum E. coli HCP F117 Goat, neat serum E. coli HCP F117-PA Goat, IgG fraction E. coli HCP AP117 Goat, affinity purified Haemophilus influenzae 8-21A Rabbit, neat serum Haemophilus influenzae 8-21A-PA Rabbit, IgG fraction Haemophilus influenzae 8-53B Guinea Pig, neat serum Haemophilus influenzae 8-53B-PA Guinea Pig, IgG fraction HEK 293 HCP HK293 Goat, neat serum Herpes Simplex Virus 8-25A Rabbit, neat serum Herpes Simplex Virus 8-25A-PA Rabbit, IgG fraction Herpes Simplex Virus 8-40B Guinea Pig, neat serum Klebsiella pneumoniae, Genus 9-01 Rabbit, neat serum Lactococcus lactic HCP LL5557 Goat, neat serum Lactococcus lactic HCP LL5557-PA Goat, IgG fraction Lactococcus lactic HCP LL5557-AF Goat, affinity purified MRC5 HCP MRC5 Goat, neat serum MRC5 HCP MRC5-PA Goat, IgG fraction MRC5 HCP MRC5-AF Goat, affinity purified Mycobacterium genus 8-27B Rabbit, neat serum Mycobacterium genus 1-04-PA Guinea Pig, IgG fraction Neisseria meningitidis 8-24B Rabbit, neat serum Neisseria meningitidis 8-36 Guinea Pig, neat serum NS/0 HCP N9192 Goat, neat serum NS/0 HCP N9192-PA Goat, IgG fraction NS/0 HCP N9192-AF Goat, affinity purified PER.C6 cell line HCP PC118 Goat, neat serum PER.C6 cell line HCP PC118-PG Goat, IgG fraction PER.C6 cell line HCP PC118-AF Goat, affinity purified Pichia pastoris HCP PI-49 Goat, neat serum Pichia pastoris HCP PI-49PA Goat, IgG fraction Pichia pastoris HCP PI-49AF Goat, affinity purified Pichia pastoris HCP PI-49AF-B Goat, affinity purified-biotinylated Pichia pastoris HCP PI-49AF-AP Goat, affinity purified-alk. phosphatase Pseudomonas fluorescens 7-04 Goat, neat serum Pseudomonas fluorescens 7-04PA Goat, IgG fraction Pseudomonas fluorescens 7-04AF Goat, affinity purified Pseudomonas species 8-57 Guinea Pig, neat serum Saccharomyces cerevisiae HCP 82-SC Goat, neat serum Saccharomyces cerevisiae HCP 82SC-PA Goat, IgG fraction Saccharomyces cerevisiae HCP 82SC-AF Goat, affinity purified Shigella species 9-03-PA Rabbit, IgG fraction Shigella species 9-04 Rabbit, neat serum SF9 HCP 12-SF9 Rabbit, neat serum SF9 HCP 12-SF9-PA Rabbit, IgG fraction SF21 HCP 34-SF21 Rabbit, neat serum SF21 HCP 34-SF21-PA Rabbit, IgG fraction SP2/0 HCP SP110 Goat, neat serum SP2/0 HCP SP110-PA Goat, IgG fraction SP2/0 HCP SP110-AF Goat, affinity purified SP2/0 HCP SP110AF-B Goat, affinity purified-biotinylated SP2/0 HCP SP110AF-AP Goat, affinity purified-alk. phosphatase S. aureus HCP SA8114 Goat, neat serum S. aureus HCP SA8114-AF Goat, affinity purified Streptococcus pneumoniae 8-31B Rabbit, neat serum Streptococcus pneumoniae 8-42A Guinea Pig, neat serum Streptococcus pneumoniae 8-43A-PA Guinea Pig, IgG fraction Total Goat Milk Proteins 4042 Rabbit, neat serum Total Goat Milk Proteins 4042-PA Rabbit, IgG fraction Total Goat Milk Proteins 4042-AF Rabbit, affinity purified Vero cell HCP VC807 Goat, neat serum Vero cell HCP VC807-PA Goat, IgG fraction Vero cell HCP VC807-AF Goat, affinity purified Stab-ELISA-rÔ Enzyme Conjugate Diluent/Stabilizers Alkaline Phosphatase I-045 500mL Alkaline Phosphatase I-040 1000mL Alk Phos w/ HAMA blocker I-045H 500mL Alk Phos w/ HAMA blocker I-040H 1000mL Horseradish Peroxidase I-035 500mL Horseradish Peroxidase I-030 1000mL HRP w/ HAMA blocker I-035H 500mL HRP w/ HAMA blocker I-030H 1000mL Liquid Substrates PNPP Liquid Substrate F008 For use with Alk Phos in ELISA, 100mL PNPP Liquid Substrate F10008 For use with Alk Phos in ELISA, 1L TMB Liquid Substrate F027 For use with HRP in ELISA, 100mL TMB Liquid Substrate F100027 For use with HRP in ELISA, 1L TMB Liquid Substrate F129 For use with HRP in Western Blot, 100mL TMB Liquid Substrate F100029 For use with HRP in Western Blot, 1L BCIP/NBT Liquid Substrate F064 For use with Alk Phos in WB, 1 reagent, 100mL BCIP/NBT Liquid Substrate F00064 For use with Alk Phos in WB, 1 reagent, 1L Sample Dilution Buffers Sample Dilution Buffer, 1L， 100mL, 500mL I028 I028-100 I028-500 For use with BHK, E.coli, CHO, CHO-CM, Human IgG's, HSA, hTransferrin, HEK 293, Insulin, L.lactic, Mouse IgG's, Pichia pastoris, S.cerevisiae, Protein A, Vero cell ELISA kits A549 Sample Diluent, 100mL , 500mL F233A-100 F233A-500 For use with A549 HCP ELISA kit BSA Sample Dilution Buffer, 1L , 100mL , 500mL F031A / F031A-100 / F031A-500 For use with BSA, Bovine IgG, Bovine Plasma Proteins and Bovine Transferrin ELISA kits Goat IgG Dilution Buffer, 1L , 100mL , 500mL F213A /F213A-100 / F213A-500 For use with Goat IgG ELISA kit Goat Milk Diluent Buffer, 1L , 100mL r, 500mL F243A /F243A-100 /F243A-500 For use with Goat Milk Proteins ELISA kit NS/0 Sample Dilution Buffer, 1L ，100mL ，500mL F223A / F223A-100 /F223A-500 For use with NS/0 HCP ELISA kit SP2/0 Sample Dilution Buffer, 1L , 100mL , 500mL F183A / F183A-100 / F183A-500 For use with SP2/0 HCP ELISA kit Immunogenicity Products Passive Coated Immunogenicity Kit IM10 Kit for immunogenicity testing Biotinylated Antigen Coated Immunogenicity Kit IM20 Kit for immunogenicity testing Biotinylation Kit IM30 Human Immunoglobulin Screening Kit IM40 Kit for immunogenicity testing Human IgG Subclass Kit IM50 Kit for immunogenicity testing Substrate Kit IM109 TMB & Stop Solution for ELISA, 50mL Coating Buffer, 1L IM101 For immunogenicity testing Conjugate Diluent, 1L IM103 For immunogenicity testing Conjugate Diluent, 500mL IM103-500 For immunogenicity testing Sample Diluent, 500mL IM104 For immunogenicity testing FG Blocker, 1L IM107 For immunogenicity testing BSA Antigen, 1mg/mL IM112 For immunogenicity testing Bovine IgG Antigen, 1mg/mL IM113 For immunogenicity testing Bovine Transferrin Antigen, 1mg/mL IM114 For immunogenicity testing CHO HCP Antigen, 1mg/mL IM115 For immunogenicity testing E. coli HCP Antigen, 1mg/mL IM116 For immunogenicity testing Goat IgG Antigen, 1mg/mL IM117 For immunogenicity testing HSA Antigen, 1mg/mL IM118 For immunogenicity testing Human IgD Antigen, 1mg/mL IM141 For immunogenicity testing Human Transferrin Antigen, 1mg/mL IM119 For immunogenicity testing Insulin Antigen, 1mg/mL IM121 For immunogenicity testing Mouse IgG Antigen, 1mg/mL IM122 For immunogenicity testing NS/0 HCP Antigen, 1mg/mL IM123 For immunogenicity testing SP2/0 HCP Antigen, 1mg/mL IM125 For immunogenicity testing Gyros Kits-discontinued CHO 3G HCP G550 For use on the Gyros xP workstation, 560 tests CHO 3G HCP G550-1 For use on the Gyros xP workstation, 112 tests E.coli HCP G410 For use on the Gyros xP workstation, 560 tests E.coli HCP G410-1 For use on the Gyros xP workstation, 112 tests Protein A G400 For use on the Gyros xP workstation, 560 tests Protein A G400-1 For use on the Gyros xP workstation, 112 tests DNA Kits DNA Extracton in Wells D100W 96 tests in a deep well microtiter plate DNA Extraction in Tubes D100T 50 tests in microfuge tubes Residual CHO Host Cell kit in Wells D550W 96 tests in a deep well microtiter plate Residual CHO Host Cell kit in Tubes D550T 50 tests in microfuge tubes CHO Amplification kit in Wells D555W 96 tests in a deep well microtiter plate CHO Amplification kit in Tubes D555T 50 tests in microfuge tubes Residual Ecoli Host Cell kit in Wells D410W 96 tests in a deep well microtiter plate Residual Ecoli Host Cell kit in Tubes D410T 50 tests in microfuge tubes E.coli Amplification kit in Wells D415W 96 tests in a deep well microtiter plate E.coli Amplification kit in Tubes D415T 50 tests in microfuge tubes Residual NS/0 Host Cell kit in Wells D220W 96 tests in a deep well microtiter plate Residual NS/0 Host Cell kit in Tubes D220T 50 tests in microfuge tubes Miscellaneous Reagents & Components Deep Well Extraction Plate w/Sealing Mat D102 5 plates DNA Extraction Buffer for use in tubes D108 30mL DNA Extraction Buffer for use in wells D105 30mL DNA Sample Diluent D006-100 100mL DNA Sample Diluent D006-500 500mL DNA Sample Diluent D006-1000 1L DNA TE Buffer, 1L D001 1L CHO DNA Concentrate D552 10ug/mL, 1mL E.coli DNA Concentrate D412 10ug/mL, 1mL NS/0 DNA Concentrate D222 10ug/mL, 1mL 更多詳情請來電: : 重慶市華雅干細(xì)胞技術(shù)有限公司 |
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